Mai Nguyet Thu
Hong1, Tran The Trong1, Tran Thi Thao1, Lam
Thi To Nga1, Tran Nguyen Van Anh1,
Hoang Kim Loan1, Ly Phi Phuïng1, Tran Thanh Son1,
Ha Ba Khiem1, Nguyen Thi Hoi1,
Truong Thi Xuan Lien1, Doan
Thò Tam2, Pham Duy Linh3, Le Hoang San4,
Nguyen Nhu Mai4.
Marina Gheorghiu5, Lagranderie Micheline5, Gilles
Marchal5.
1Pasteur Institute
in
3 Pham Ngoc Thach
Tuberculosis and lung diseases Center,
4 Medicine Preventive
Center of TienGiang Province, 5Pasteur
Institute in
BCG vaccine is the only vaccine with the others methods for tuberculosis prevention such as: improving of environment hygiene, resistant enhancement to tuberculosis in high risk group, good management and separation of tuberculosis patients. Since, the observation of the characteristics of this vaccine is very necessary, specially, the long period observations.
This study observed the characteristics of BCG vaccine produced in Pasteur Institue of HoChiMinh city from 1990 to 2000. This was the period that the Minister of Health and “Amico-Amitié Franco-Vietnamnienne” decided to set up a new BCG laboratory, and at that time, this BCG laboratory had been received the technical transmitting of BCG vaccine production from Pasteur Institute of Paris.
In ten years of production, with 2 to 4 million of doses per
year, the vaccine responds to the requirements of
-
The in vitro test demonstrated that the
viable counts varied from 2 to 3 millions colony forming units per mg of
vaccine. The opacity at 490 nm varied from 0.185 to 0.249 and the diffusion index
of vaccine varied from 1.16 to 1.37.
-
The in vivo test showed that the 510 vaccine
lots were not absolutely toxic and had a good effectiveness in 6120 guinea
pigs.
-
Vaccine
gave good immunity and the low secondary reaction in human. 94.11% of newborns and
100% of children up 3 months had an immunity after
vaccination. 60.25% of these individuals remained this immunity in 5 years and
60.10% in 10 years.
-
The
lymphadenitis suppurative rate was 0.98% in newborns and 0% in children up 3
months.
-
The
positive transfer reaction after BCG revaccination was high. 52.11% before
revaccination and 96.17% after revaccination.
-
Not any
secondary reaction or complication was observed, even in the sensitivity to
tuberculin individuals before revaccinatin.
-
Vaccine
had a good protection. 89.72% of newborns in HoChiMinh city had an protective ability against to tuberculosis after BCG
vaccination (a report of Tuberculosis and
-
An molecular
observation demonstrated that the BCG had the IS 6110 gene. This was the
specific sequence of M. tuberculosis complex. This structure was similar to
the other observations had been published.
These results demonstrated that the BCG vaccine produced in
Pasteur Institue of HoChiMinh city from 1990 to 2000 responded to the
requirements of