APPLICATION OF THE DISPERSED CULTURE METHOD IN BCG VACCINE PRODUCTION

Tran Thi Thao,Tran The Trong, Tan Thi Ngoc Bich, To Cam Nhung
Nguyen Thi Kim Dung, Mai Nguyet Thu Hong, Tran Thanh Son, Ly Phi Phung

Pasteur Institute in HCM City

To improve the better technique, raise the vaccine quality. Since then we can determine the exact dose to get the high protection and reduce the purulent ganglion when injection.

Pasteur Institute in HCM City has succeed to produce BCG vaccine freeze-dried by dispersed culture method:

All examination test give the good result, as follows.

– Alive vaccine level fresh: 91.72 ± 9.20 x 10⁶ CFU/mg.

– Alive level after freeze-dried: 39.90 ± 6.20 x 10⁶ CFU/mg.

– % alive after freeze-dried: 43.84 ± 7.78 x 10⁶ CFU/mg.

– % stability with temperature: 32.72 ± 4.13 x 10⁶ CFU/mg.

– Dispersive level: 1.78 ± 0.09 x 10⁶ CFU/mg.